Assessment of Cytotoxic Effects of Latex from <i>Cascabela thevetia</i> (L.) Lippold and <i>Plumeria alba</i> L. via <i>In vitro</i> and <i>In silico</i> Approaches

Plant latex has been found to occur in more than 40 families and among them, Apocynaceae is one. Two plants of this family i.e., Cascabela thevetia (L.) Lippold Plumeria alba L. had chosen for the current experimental work. The aqueous methanolic extracts were evaluated their phytochemical constituents cytotoxic activities. To determine effects extracts, MTT [3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide] assay was performed by using cell lines A549 Human Lung Cancer MCF-7 Breast Cancer. Further, confirmation apoptosis conducted. richness determined GC-MS HPTLC methods. preliminary analysis conducted methodology. presence cardiac glycosides analyzed High-Performance Thin Layer Chromatography. Here, Digoxin used as standard. Studies have revealed that Na+ K+-ATPase can serve a powerful target developing anticancer drugs exhibited via inhibition K+-ATPase. Hence, molecular docking studies which 6KPX served compounds NIST library ligands. Molecular confirmed best based on RF score, binding affinity, binder whether these good or bad. These methodologies altogether helped evaluating plant potent molecules present them.